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The experimental system that permits observation of rotation in the F1 component of F1F0 ATP synthas

Description
The cloned gene encoding the F1b subunit was modified by adding a sequence that encodes an oligohistidine sequence that binds to a nickel-coated bead (Ni-NTA coated bead) in the conformation shown.

Thus, the complex was immobilized on the bead and attached to a glass coverslip.

Streptavidin is a protein used to couple fluorescent-tagged actin to the subunit.
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