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Chromosome structural changes that accompany dihydrofolate reductase gene amplification
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Description
The micrograph shows metaphase chromosomes from Chinese hamster ovary cells that are highly resistant to methotrexate.

Chromosomal DNA was subjected to hybridization in situ with a fluorescence-labeled DHFR gene probe.

White arrows point to single-copy genes.

The amplified chromosomal sequences are on a giant form of the chromosome that also contains one of the original single-copy sequences.
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