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Enzymatic synthesis of DNA

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(a) DNA polymerase recognizes free nucleotides called deoxynucleoside triphosphates and attaches a deoxynucleoside monophosphate to the 3? end of a growing strand. (b) DNA polymerase breaks the bond at the middle phosphate in a deoxynucleoside triphosphate, causing the release of pyrophosphate. This provides the energy to form a covalent phosphoester bond between the resulting deoxynucleoside monophosphate and the previous nucleotide in the growing strand.
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