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DNA Cloning
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Description
An example of cloning. Here, a fragment of chromosomal DNA is inserted into a plasmid.

A A restriction enzyme cuts
a specific base sequence in chromosomal DNA and in
a plasmid cloning vector.

B A fragment of chromosomal DNA and the plasmid base-pair at their sticky ends. DNA ligase joins the two pieces of DNA.

C The recombinant plasmid is inserted into a host cell. When the cell multiplies, it makes multiple copies of the plasmids.
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