A plasmid cloning vector

(a) MCS restriction sites for cloning M13 forward- Psfl BamHl M13 reverse- sequencing ' Smal Sacl sequencing primer Hindlll Pael Acc65l primer 5' GTMMCGACGGCCA CATMSCTG‘I‘ITCCTG 3' 3’ CATFITGCTGCCGGT AA ATCGACMAGGAC 5' ‘~\\ - ,,/’ “~\c\ MCS ,,”’ The selectable marker gene for B-lactamase, b/a (AmpR), confers resistance to ampicil- lin. The origin of replica- tion allows DNA replication in bacteria. (b) White colonies identify bacteria in which the lacZ gene is disrupted, and thus contain recombinant DNA molecules. In a medium containing X-gal, blue colonies identify bacteria with functional lacZ gene.