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Electron micrographs of a cellular membrane.

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(a) In TEM, the membrane appears as two dark parallel lines. These lines are the lipid head groups stained with osmium tetroxide. The fatty acyl tails do not stain well and appear as a light region sandwiched between the dark lines.

(b) In FFEM, a sample is frozen in liquid nitrogen, split, and fractured. Membranes that happen to be located at the fractured region will split along their leaflets. The sample is then coated with metal and viewed with a scanning electron microscope.
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