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RESEARCH TECHNIQUE
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DNA Footprint Protection Assay 0 Control 9 Experimental Identical DNA End-label DNA with 32P 32p — End~labe|ed DNA No protein added to DNA 1 Transcriptional protein 1 complex added to DNA ,,,,,,,,,,,,,,,, L 1' WDNase i added: cleaves —DNase I added: cleaves ‘ ““P'Oteded DNA 998E9l99€qu9§fiwsn 1 1111 111 m-) llllllllll i Gel 32P—labeled fragments e|ectrophoresi5”P-labeled fragments l l Protein-protected region; potential promoter region Bound transcriptional protein protects promoter region from enzymatic cleavage.
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