Deletion mapping of mutants in the rll region

Wild-type recombinants form if the site of point mutation does not overlap the site of deletion, but if the two mutation sites overlap, no wild-type recombinants are possible Wild-type recombinants form if the site of point mutation does not overlap the site of deletion, but if the two mutation sites overlap, no wild-type recombinants are possible

(a) Nonoverlapping mutations, (b) Overlapping mutations, wild-type recombination no wild-type recombinants rll region rll region A B A B A B A B :I--|--:Ex:—:E J-r-:EX:_:E Deletion Point Deletion Point mutation mutation mutation mutation Coinfection Coinfection l l A B A | B Recombination Recombination A B A B A B A B :I---: and 2-:l: :I--:l: and -:l: Double mutant Wild type Deletion mutant Point mutant recombination to generate wild-type type recombinants are produced. Nonoverlapping mutations allow ‘ The mutations overlap and no wild— I recombinants and double mutants.