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7 years ago
Antisense mRNA can be used to regulate the expression of genes. Briefly describe how this technology was used to slow the ripening of Calgene's ill-fated Flavr-SavrTM tomato.
 
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7 years ago
In engineered tomatoes, the gene for polygalacturonase, the enzyme responsible for fruit softening, was inserted backwards. This yielded an antisense mRNA copy that bonded with the sense mRNA, reducing the effective transcript level present for translation. Ripening was thus retarded relative to the normal tomato.
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