× Didn't find what you were looking for? Ask a question
Top Posters
Since Sunday
e
4
h
4
h
4
m
3
d
3
B
3
o
3
w
3
H
3
a
3
c
3
k
3
Home Q & A Board Biology-Related Homework Help Cell Biology
New Topic  
sma1394 sma1394
wrote...
Posts: 2
Rep: 0 0
11 years ago
Theoretically, there should be no uptake, but when I did my results, the bacteria had indeed taken up the plasmid DNA even when I didn't do any heat shock?

Does anyone have any explanations for this?
Read 528 times
3 Replies

Related Topics

Replies
wrote...
#1
11 years ago
Maybe you added chemical reagents that help make the membrane permeable? I think calciumchloride can do that.

EDIT: Or it might be that you already have bacteria with the altered plasmid. They can exchange their plasmids which then is viewed by you as 'uptake'.
wrote...
#2
11 years ago
Transformation - uptake of plasmids, is commonly induced by calcium chloride.
wrote...
#3
11 years ago
Yes they can, but much less efficiently.  Sharing plasmids between different species or even different genera has been documented in nature.
New Topic      
Quick Reply
Explore
Post your homework questions and get free online help from our incredible volunteers
  1032 People Browsing
Start New Topic Take the Tour CoursesNew Study Tools Topics Trending Browse by Textbook
Live Chat
Related Images
  
 111
  
 1001
  
 815
Your Opinion
Which 'study break' activity do you find most distracting?
Votes: 824
Previous poll results: Do you believe in global warming?