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Home Q & A Board Biology-Related Homework Help Cell Biology
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Katty2 Katty2
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6 years ago
With fully automated Sanger sequencing, all four chain-terminating ddNTPs can be added into a single reaction. This is different from the traditional slab gel Sanger sequencing, where a different reaction had to be carried out for each ddNTP.
 
  The mixing of all four ddNTPs can be carried out because ______________.
  (a) the fully automated Sanger sequencing reactions are loaded onto a capillary gel.
  (b) the fully automated Sanger sequencing reactions utilize ddNTPs each labeled with a different fluorescent tag, which allows all four ddNTPs to be incorporated into a single molecule of DNA.
  (c) the fully automated Sanger sequencing reactions generate a set of products, each of which carries a single fluorescent tag whose color reveals the identity of the base that is at the end of the product.
  (d) the fully automated Sanger sequencing reactions do not require DNA polymerase because the bases are read as the DNA is pulled through a tiny pore at the end of the capillary gel.
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salmajed1salmajed1
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Katty2 Author
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6 years ago
Thanks for your help!!
kobbhy
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Yesterday
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brina10
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2 hours ago
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