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920073545 920073545
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9 years ago
Sequence:
5'ATCGATGTGCTATACATGTTAACAC3'
3'TAGCTACACGATATGTACACTTGTG5'

In order to amplify DNA sequences, should I always use the first six of the top strand and the last six of the bottom strand? So in this example, the two primers should be 5'ATCGAT3' and 3'CTTGTG5' ?

Thank you!
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Educator
9 years ago Edited: 9 years ago, bio_man


Watch this video starting at 3:30. It will help you with your current problem.

Good luck.
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