Mitochondria Experiment. What to study?

 Hello researchers and science lovers,

   My question involves mitochondria. Specifically mitochondrial metabolism.
 I have found a compound that, i believe, disrupts metabolism of mitochondrias. However my question for everyone, patient enough to answer, is how can i study and confirm this disruption that i believe this compound do in mitochondria. I can do some oxygen electrode some may think. But what about proteins in study? Can i take a specific protein and bind it with something to see? And then what protein do you guys suggest? Because in mammalian mitochondria there is more than 1000 proteins..

  Thanks and i hope i get some ideas.