× Didn't find what you were looking for? Ask a question

Recent Posts • Latest Topics • Unanswered Questions

Nile Red Membrane Staining Protocol

micro

wrote...

Posts: 170

Rep:

11 years ago

Nile Red Membrane Staining Protocol

Ever encountered a good protocol to stain bacterial cell membrane with nile red?

Read 2698 times

8 Replies

Report

Related Topics

Solved Annexin-V staining of cells identifies: a)a failure of membrane integrity. b)
Solved Biosafety Protocol is a United Nations-sponsored protocol that requires signator
The Kyoto Protocol is to carbon dioxide as the Montreal Protocol is to ________.
Solved The IPv4 protocol is beginning to be replaced by the ________ protocol.
Solved Unlike the Web protocol, the ________ protocol is designed solely to allow files to be transferred f
Solved In the Hymn to the Nile, the Nile is compared to which of the following?
Solved Annexin-V staining of cells identifies
Direct immunofluorescence staining of a biopsy from a patient with mucous membrane pemphigoid would ...

Replies

padre

wrote...

#1

Staff Member

Educator

11 years ago

HI micro, sorry for the late reply.

Attached file

Nile_Red_staining_for_lipids.doc (20 KB)

You must login or register to gain access to this attachment.

Report

Mastering in Nutritional Biology
Tralalalala Slight Smile

micro Author

wrote...

#2

11 years ago

Thank you very much! When it says to place a drop of the suspension and leave it in a damp environment, does it have to dry up before staining?

Report

padre

wrote...

#3

Staff Member

Educator

11 years ago

HI micro, are you talking about step #4?

Place 100 ul drops on coverslips and place in tray with dampened paper towel and cover until needed.

Report

Mastering in Nutritional Biology
Tralalalala Slight Smile

micro Author

wrote...

#4

11 years ago

Correct

Report

padre

wrote...

#5

Staff Member

Educator

11 years ago

Don't let it dry.

Report

Mastering in Nutritional Biology
Tralalalala Slight Smile

micro Author

wrote...

#6

11 years ago

When it comes to the dilution (x100); can it be diluted further into acetone? The 3 papers I read seem to use nile red in acetone or DMSO.

Report

padre

wrote...

#7

Staff Member

Educator

11 years ago

Quote from: micro (11 years ago)

The 3 papers I read seem to use nile red in acetone or DMSO.

Nile red is usually stored in acetone, isn't it?

Report

Mastering in Nutritional Biology
Tralalalala Slight Smile

katerina2005

wrote...

#8

5 months ago

TY :)

Report

New Topic

Quick Reply

Explore

Post your homework questions and get free online help from our incredible volunteers

1255 People Browsing

Start New Topic Take the Tour CoursesNew Study Tools Topics Trending Browse by Textbook

Related Images

1797

1909

120

Your Opinion

Previous poll results: Who's your favorite biologist?

It's sore throat season, why does my mucus have red spots?

The Toxic Skin and Fungi Defense

Strange Ingredients Lurking in Doritos Chips

Courses by Biology Forums

Bonobos, Chimpanzees, and the 98% DNA Link

Ready to ask a question on Biology Forums? Try it out

Join Our Community

Biology Forums - Study Force is a free online homework help service catered towards college and high school students. Get homework help and answers to your toughest questions in biology, chemistry, physics, mathematics, engineering, accounting, business, humanities, and more. Master your assignments quickly with thousands of step-by-step solutions to countless textbook questions asked and answered by our members. If we don't have your question, don't worry. You can ask any homework question and get expert homework help in as little as two hours.

Our extensive online study community is made up of college and high school students, teachers, professors, parents and subject enthusiasts who contribute to our vast collection of study resources: textbook solutions, study guides, practice tests, practice problems, lecture notes, equation sheets and more. With our help, your homework will never be the same!

Join for free today

How is Biology Forums - Study Force different than tutoring?

Home Search Q & A Board Gallery Resource Library Blog Dictionary Chat	Quick Links Start a Topic Latest Topics Unanswered Questions Courses Study Tools Browse by Textbook	Social Facebook YouTube Community Stats Who's Online Trending Staff	Help About Us Take the Tour Study Tips Posting Guidelines Terms and Policies Contact Us
Biology Forums - Study Force © 2010-2025 \| Powered by SMF \| SMF © 2015, Simple Machines \| Sitemap Biology-Forums.com is not affiliated with any publisher. Book covers, title and author names appear for reference only.