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Home Q & A Board Biology-Related Homework Help Cell Biology
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ankochan ankochan
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10 years ago
How could you determine whether the turbidity in your nutrient broth tube was from a mixture of  different microbes or from the growth of only one kind of microbe?
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#1
Staff Member
Educator
10 years ago
The simplest method is to use a sterile pipette to take a small amount from the turbid mixture, mix it with distilled water and view under microscope. Use simple stain 1% solution of methylene blue/carbol fuschin/saffranin/crystal violet. you can easily identify if there are different microbes by shape,size,staining property.

Good luck
Mastering in Nutritional Biology
Tralalalala Slight Smile
wrote...
#2
10 years ago
You can't tell from looking. you'll have to streak a plate and look at the colonies formed and their morphology. If all the colonies look the same, chance favors a pure culture. However, you could do a few stains from the broth to examine if the microbes look different

Gram Stain comes to mind immediately because you don't need to have a pure culture to get good results.
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wrote...
#3
10 years ago
You could transfer the sample to a Petri dish with agar and see if different types of colonies grow
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