Gene funtion mutated in each defect during replication

In E. coli this protein is DnaA.

Sequences used by initiator proteins tend to be "AT-rich" (rich in adenine and thymine bases), because A-T base pairs have two hydrogen bonds (rather than the three formed in a C-G pair) which are easier to unzip.


The gene mutation here would be mismatch repair endonucleases not functioning correctly (not 100% sure). It might also be DNA polymerase.


DNA polymerase I -- In E. coli, which DNA polymerase is responsible for the removal of the RNA primers at the origin and at the 5' ends of the Okazaki fragments. The 5' to 3' exonuclease activity of DNA polymerase I is responsible for RNA primer removal.


-5' to 3' exonuclease activity removes Okazaki fragments
-5' to 3' polymerase activity to fill gap