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Home Q & A Board Laboratory Help Upper-Year Courses
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olij olij
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9 years ago
I am working on pectinase enzyme assay. I incubated 900 ul of substrate for 10 minutes in the water bath, followed by adding 2ml of DNSA reagent, then 100ul of enzyme extract added finally i read the absorbance @ 540 OD. However the values are high. How can I troubleshoot high enzyme blank values in Pectinase assay ?
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#1
Staff Member
9 years ago
Quote from: olij (9 years ago)
then 100ul of enzyme extract added finally i read the absorbance  540 OD.

What was the absorbance prior to adding the enzyme?
- Master of Science in Biology
- Bachelor of Science
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