(Solved) Restriction enzyme digestion with XbaI? (Due tomorrow!!)

darwincalledit

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9 years ago

Restriction enzyme digestion with XbaI? (Due tomorrow!!)

Hello! I'm having a hard time placing the XbaI restriction enzyme on the gel (attached). An explanation would be awesome!! Slight Smile

Part B
How many fragments will be formed by restriction enzyme digestion with XbaI in the linear form of the λ genome?
   A: 1
   B: 2
   C: 3
   D: 4

Part C
How many fragments will be formed by restriction enzyme digestion with both XhoI and XbaI in the linear form of the λ genome?
    A:1
    B:2
    C:3
    D:4

Part D
How many fragments will be formed by restriction enzyme digestion with XhoI in the circular form of the λ genome?
    A:1
    B:2
    C:3
    D:4

Part E
How many fragments will be formed by restriction enzyme digestion with XbaI in the circular form of the λ genome?
    A:1
    B:2
    C:3
    D:4

Part F
How many fragments will be formed by restriction enzyme digestion with both XhoI and XbaI in the circular form of the λ genome?
    A:1
    B:2
    C:3
    D:4

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bio_man

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Educator

9 years ago

Quote from: darwincalledit (9 years ago)

Part B
How many fragments will be formed by restriction enzyme digestion with XbaI in the linear form of the λ genome?
A: 1
B: 2
C: 3
D: 4

Try 2.

Quote from: darwincalledit (9 years ago)

Part C
How many fragments will be formed by restriction enzyme digestion with both XhoI and XbaI in the linear form of the λ genome?
A:1
B:2
C:3
D:4

Quote from: darwincalledit (9 years ago)

Part D
How many fragments will be formed by restriction enzyme digestion with XhoI in the circular form of the λ genome?
A:1
B:2
C:3
D:4

1

Let me know how these pan out.

2 Votes

ray23, jjj716

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darwincalledit Author

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Posts: 17

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9 years ago

1 Vote

bio_man

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coolman2013

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9 years ago

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KobeLee

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nisha2cute

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thanks

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Vanessa753

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#10

9 years ago

Thank You

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odyan

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#11

9 years ago

This is my answer:
Part A
How many fragments will be formed by restriction enzyme digestion with XhoI in the linear form of the λ genome? (2)
Part B
How many fragments will be formed by restriction enzyme digestion with XbaI in the linear form of the λ genome? (2)
Part C
How many fragments will be formed by restriction enzyme digestion with both XhoI and XbaI in the linear form of the λ genome?(3)
Part D
How many fragments will be formed by restriction enzyme digestion with XhoI in the circular form of the λ genome?(1)
Part E
How many fragments will be formed by restriction enzyme digestion with XbaI in the circular form of the λ genome?(1)
Part F
How many fragments will be formed by restriction enzyme digestion with both XhoI and XbaI in the circular form of the λ genome?(2)

1 Vote

nyq87

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nyq87

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#12

9 years ago

Quote from: odyan (9 years ago)

thank you!

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