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Home Q & A Board Biology-Related Homework Help Genetics and Developmental Biology
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aphalfi aphalfi
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6 years ago
Nucleic acid blotting is commonly used in molecular biology. Two types, Southern blots and Northern blots, involve gel electrophoresis and a filter, which holds the nucleic acid.
 
  Briefly describe the procedure of blotting in this context and differentiate between Southern and Northern blots.
Textbook 
Essentials of Genetics

Essentials of Genetics


Edition: 9th
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6 years ago
In a Southern blot, the DNA to be probed is cut with a restriction enzyme(s); then the fragments are separated by gel electrophoresis. Alkali treatment of the gel denatures the DNA, which is then blotted onto the filter (nylon or nitrocellulose). A labeled probe (RNA or DNA) is then hybridized to complementary fragments on the filter. In a Northern blot, RNA is separated in the gel and probed with the labeled DNA.
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