What are some key differences in replication between E. coli DNA and phage DNA? What will be an ...

Both start replication as a circular molecule, and in E. coli, the parental DNA strands remain in a circular form throughout the replication cycle. A replication bubble opens to form two replication forks, and replication proceeds bidirectionally. Lambda phage DNA is replicated by a rolling circle mechanism, in which a nick is made in one of the two strands of the circle, and the 5' end of the cut DNA strand is rolled out as a free tongueof increasing length as replication proceeds. The parental DNA circle is replicated continuously, while the linear displaced strand is replicated discontinuously. As long as the circle continues to roll, concatamers of phage DNA can be produced. This is later cut up into linear chromosomes and packaged into new phage heads.