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Home Q & A Board Biology-Related Homework Help Genetics and Developmental Biology
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Salmams Salmams
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6 years ago
How could you use T2 bacteriophage plaque phenotypes to determine the genetic distance between two genes, h and r, that affect plaque appearance in the phage DNA?
 
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6 years ago
Just as in eukaryotic organisms, a crossing experiment can be performed. You would use different strains of T2one of which is h+r and one of which is hr+that express different plaque phenotypes to coinfect plated bacterial cells. Inside the bacterial cells, the two different phage chromosomes can crossover to produce recombinants. The percentages of parental and recombinant plaque phenotypes can be determined. As in eukaryotic cells, the frequency of recombinants is equal to the genetic distance between the two genes.
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