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Home Q & A Board Biology-Related Homework Help Genetics and Developmental Biology
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koala423 koala423
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6 years ago
Your student is trying to create a Gene X knockout mouse by introducing a recombinant construct into embryonic stem (ES) cells, selecting transformants, and introducing the transformed cells into mouse blastocysts.
 
  He designed his construct as follows: NeoR----region of homology to Gene X---HSV tk--- region of homology to Gene X where NeoR is the neomycin-resistance gene and HSV tk is the Herpes Simplex virus thymidine kinase gene. He transforms the ES cells and exposes them to G418 and ganciclovir. Explain to your student he failed to recover transformed ES cells.
Textbook 
Genetic Analysis: An Integrated Approach

Genetic Analysis: An Integrated Approach


Edition: 3rd
Authors:
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Beula74Beula74
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koala423 Author
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6 years ago
Correct Slight Smile TY
amandamraz
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Yesterday
this is exactly what I needed
ethom947
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2 hours ago
Helped a lot
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