Which of the following is true of the Sanger sequencing method?
A. It requires an RNA template to copy.
B. It incorporates dNTPs using an RNA polymerase.
C. It requires a primer for the polymerase; the primer is radiolabelled so the sequences generated can be visualized.
D. It requires chain terminating ddNTPs which lack a 2'OH and are thus unable to form a bond with the next nucleotide.
E. The sequencing reaction products are separated by affinity chromatography.
During the process of eukaryotic mRNA degradation, all of the following occur except:
A. The poly(A) tail is lengthened.
B. The 5' cap is removed.
C. The exosome degrades the molecule to component nucleotides.
D. Localization to processing bodies occurs.
How do modified and unmodified histone subunits become distributed among nucleosomes after replication?
A. The newly synthesized DNA strand contains unmodified histone subunits, while the old strand retains the modified histones.
B. Both strands of DNA are repopulated first with a mixture of modified and unmodified H2A histones, followed by mixtures of H2B, H3, and, finally, H4.
C. Both strands of DNA are repopulated first with a mixture of modified and unmodified H2A and H2B histones, followed by mixtures of H3 and finally mixtures of H4.
D. Both strands of DNA retain H3-H4 modified pairs, and then are repopulated with unmodified H3-H4 pairs, followed by modified and unmodified H2A-H2B.
E. Both strands of DNA retain H2A-H2B modified pairs, and are then repopulated with unmodified H2A-H2B pairs and then mixtures of modified and unmodified H3-H4 pairs.
Which of the following nuclear RNA transport events requires the action of a guanine exchange factor (GEF)?
A. importin release of RNA in the nucleus
B. exportin release of RNA in the cytoplasm
C. importin binding of RNA in the cytoplasm
D. exportin binding of RNA in the nucleus
The PABP bound to the poly(A) tail and eIF4E bound at the 5' UTR interact through their binding to:
A. the 40S ribosomal subunit.
B. eIF4G.
C. the initiating tRNA.
D. eIF3.
Quick Reply
