How does the scanning electron microscope differ from the transmission electron microscope?

How does the scanning electron microscope differ from the transmission electron microscope?
  a. The SEM can view a live specimen.
  b. The SEM can view the internal structure of a cell.
  c. The SEM utilizes a beam of light that passes through the specimen.
  d. The SEM gives a three dimensional image of the object being studied.
  e. The SEM relies on the detection of electrons from the beam after contact with the specimens.

Question 2

You want to determine the location of a specific protein in a cell using a colored stain. Which of the following is the
  best technique for this purpose?
  a. electron microscopy
  b. phase contrast microscopy
  c. bright-field microscopy
  d. fluorescence microscopy
  e. dark-field microscopy