28. Which of the following was not one of the first uses of modern biotechnology?
Select one:
a. The insertion of a bacterial insulin gene into human cells.
b. The insertion of a human insulin gene into bacteria.
c. The cloning of a mammal.
d. The insertion of an antifreeze gene into a fish.
29. What is a recombinant DNA?
Select one:
a. During meiosis, sister chromatids cross over to produce daughter cells with recombinant DNA.
b. Any genome that is non-parental is called a recombinant DNA.
c. New DNA sequences are inserted into a genome to produce recombinant DNA.
d. Bacterial plasmid with sticky ends are referred to as the recombinant DNA.
30. Some Steps Involved in Creating Recombinant DNA
1. Plasmid DNA is cut at the restriction site.
2. Foreign DNA is cut at the restriction site.
3. Plasmid DNA is opened and sticky ends are formed.
4. Foreign DNA restriction fragments are isolated
5. Target sequence in the plasmid is recognized by the restriction endonuclease.
6. Target sequences in the foreign DNA are recognized by the restriction endonuclease.
7. Foreign DNA restriction fragment is inserted into the plasmid at the restriction site.
8. DNA ligase splices the foreign restriction fragment and the plasmid together.
Identify the correct sequence of steps involved in cutting and reassembling DNA molecules to make recombinant DNA.
Select one:
a. 5, 1, 3, 6, 2, 4, 7, 8
b. 6, 2, 4, 5, 1, 3, 8, 7
c. 5, 1, 3, 6, 2, 4, 8, 7
d. 6, 2, 4, 7, 8, 5, 1, 3
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