When preparing a smear from a viscous and granular sample the microbiologist should:
a. add saline to the sample to thin out the specimen for smear preparation.
b. place some of the material in an enrich-ment broth and then use the broth for smear preparation.
c. use a sterile swab and roll it around in the material because it will be easier to roll the sample than to use a loop on the slide.
d. if the material is not able to be distributed using a sterile loop or swab, use a squash or crush prep to spread out sample.
Selection of culture media for specific specimens includes a variety of media for all of the following reasons except:
a. a nutritive media is included to insure growth of all potential pathogens.
b. a selective media is often included to sup-press normal microbiota or other potential contaminating organisms.
c. as enrichment media is utilized to cultivate organisms that do not grow on routine media.
d. a back-up broth is included to provide in-oculum for additional testing.
A mordant that is applied after the primary stain to bond chemically the alkaline dye to the bacterial cell wall is:
a. safranin.
b. crystal violet.
c. Gram's iodine.
d. Gram's decolorizer.
Routine microbiology cultures should include all of the following media except:
a. nonselective nutritive media.
b. highly selective specialized media such as Lwenstein-Jensen agar.
c. selective and differential media based on site of infection.
d. an enrichment or supplemental broth.
The limitation associated with utilizing the Gram stain procedure includes all of the following except:
a. over decolorizing resulting in all organisms appearing gram negative.
b. formation of crystals in the stain appearing as gram-positive rods.
c. providing potential life-saving preliminary organism characteristics.
d. variable Gram staining characteristics based on organism viability in preparation.
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