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If Single-Stranded Binding protein (SSB) isnotpresent during DNA replication, what would you expect to see?

queenmeg

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If Single-Stranded Binding protein (SSB) isnotpresent during DNA replication, what would you expect to see?

If Single-Stranded Binding protein (SSB) is not present during DNA replication, what would you expect to see?

▸ The replisome complex would not assemble on the oriC region.

▸ The DNA cannot bend, so hydrogen bonds in the 13-mer region of oriC remain intact.

▸ Helicase activity is inhibited, so DNA strands cannot be separated.

▸ SSB carries the helicase protein to the open region of DNA, so hydrolysis and strand separation will not occur.

▸ SSB prevents reannealing of the separated strands, so strands would quickly reanneal and DNA replication cannot proceed.

Textbook

Genetic Analysis: An Integrated Approach

Genetic Analysis: An Integrated Approach

Edition: 3^rd

Authors: Sanders, Bowman

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queenmeg Author

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Thanks for your help!!

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