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What is the purpose of the 3'-to-5' exonuclease activity of DNA Polymerase?

MZ Mai

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What is the purpose of the 3'-to-5' exonuclease activity of DNA Polymerase?

What is the purpose of the 3'-to-5' exonuclease activity of DNA Polymerase?

▸ Some polymerases do not have exonuclease activity.

▸ Remove mismatched nucleotides in the newly synthesized strand.

▸ Degrade excessive nucleotides on Okazaki fragments.

▸ Remove mismatched nucleotides in the template strand.

▸ Removing mismatched nucleotides at the polymerase active site increases the rate of synthesis in the 5' to 3' direction.

Textbook

Genetic Analysis: An Integrated Approach

Genetic Analysis: An Integrated Approach

Edition: 3^rd

Authors: Sanders, Bowman

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