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What must eukaryotes do to initiate transcription of a gene?

▸ Open the DNA template and then bind RNA Polymerase at the transcriptional initiation site.

▸ Recruit the transcription factors and RNA Polymerase that compose the pre-initiation complex.

▸ Recruit general transcription factors to produce an open complex and then recruit RNA Polymerase.

▸ Bind RNA Polymerase to displace histone proteins that binding DNA in the promoter region.

▸ Bind transcription factors from enhancer sequences to the RNA Polymerase.
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Genetic Analysis: An Integrated Approach


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Recruit the transcription factors and RNA Polymerase that compose the pre-initiation complex.

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