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A researcher is annotating a newly-sequenced chromosome that they constructed from contigs sequenced ...

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A researcher is annotating a newly-sequenced chromosome that they constructed from contigs sequenced ...

A researcher is annotating a newly-sequenced chromosome that they constructed from contigs sequenced from a sample of Antarctic ice. The cell/organism that the chromosome came from is not known, however it yielded a single circular chromosome. You are asked to run a BLAST search to help this researcher identify the most related organism in GenBank. What would be the best strategy?

▸ Compare the most unusual portion of this newly-discovered chromosome to al known animal genomes.

▸ Perform many careful one-by-one comparisons between the newly-discovered DNA sequence and species selected from GenBank, one at a time.

▸ Use several ~1000bp portions of the DNA sequence to search for the closest matches from among the entire available database.

▸ Look for a nearly identical match to a very small part (20-40 bp) of the chromosome from all known ice bacteria.

▸ Look for an exact match to the whole chromosome.

Textbook

Genetic Analysis: An Integrated Approach

Edition: 3^rd

Authors: Sanders, Bowman

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