hi, I'm not sure if I'm posting this in the right forum, but here my problem goes
"Below is a partial mRNA sequence from a mutant gene in E. coli. used to answer the following questions.
5'... ACCCGCCACGCC... 3'
This gene is a mutant variant that severely slows the growth of the bacteria. The normal version of the encoded protein should contain the partial amino acid sequence (Nterm...T-R-N-A...Cterm). It's known that mutant forms of tRNA synthetases can charge tRNAs with a different amino acid than expected.
If a mutant synthetase gene was introduced into the E. coli, which one could produce the normal version of the encoded protein?
multiple choice
A synthetase that charges an asparagine tRNA with histidine
B synthetase that charges an asparagine tRNA with asparagine
C synthetase that charges a histidine tRNA with asparagine
D synthetase that charges a histidine tRNA with histidine"
I already got the answer from the pearson homework software, which is an absolute nightmare with these questions, but I just don't understand it. how does changing a histidine with a asparagine not effect the protein, and how was I supposed to find the answer?
the blurb for the correct answer doesn't help me much "Since the mutant sequence (Nterm...T-R-H-A...Cterm) contains a histidine instead of an asparagine in the normal sequence (Nterm...T-R-N-A...Cterm), then a tRNA is needed that recognizes the histidine codon (CAC), but can deliver an asparagine amino acid. Therefore a mutant synthetase that charges a histidine tRNA with asparagine will be needed."
My professor refuses to talk about any of the homework questions, so some help with explaining this would be great.
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