Hello fellow biologists,
I am seeking some assistance in deciphering an agarose gel electrophoresis image. The gel is a result of a molecular biology practical involving the amplification of specific genes from mushroom DNA. Here are the essential details:
DNA was extracted from freeze-dried mushrooms and then used in quantitative PCR (qPCR) to amplify two target genes.
One of the genes is a reference gene (beta actin), which is expected to be present in a single copy per cell.
The other gene is tyrosinase (related to polyphenol oxidase activities), where we used degenerate primers potentially targeting multiple gene copies.
We used a 2% agarose gel to visualize the qPCR products and employed a DNA ladder to estimate fragment sizes.
Each lane was loaded with 10 µL of the sample, and the samples from each person were alternately loaded for the two gene targets.
The gel was run at ~70 V for approximately 60 minutes.
The image shows the gel post-run, with bands visible under UV light after staining with a fluorescent dye. I am looking to identify which bands correspond to the beta actin gene and which to the tyrosinase gene, as well as any insight you may have about anything else.
I hope this information is sufficient for an experienced eye. Could anyone help me interpret which lanes contain which gene products and what the band patterns might suggest about the gene copy number or amplification efficiency?
I was not present for the practical and thus I am having to figure this out on my own
Thank you in advance for your expertise!
I am not sure how to post the images here, but hopefully it will let me edit them in
Here are the pictures :
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