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SABRINAwang SABRINAwang
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9 years ago
I am facing problems in thawing of cells. The procedure which we follow is- we take stock from liq. N2 then though it in water bath at 37 degree. Then we take medium in falcon (5ml) then add thawed cells dropwise into it. We then centrifuge it at 1500rpm for 5 mins.
but the problem is that after thawing i am unable to get viable cells.
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doubleudoubleu
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9 years ago
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When you like a flower, you just pluck it out.
But when you love a flower, you water it daily.
The one who understands this, understands life.

- That's the difference between I like you and I love you.

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Staff Member
9 years ago
http://www.lifetechnologies.com/ca/en/home/references/gibco-cell-culture-basics/cell-culture-protocols/thawing-cells.html

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