× Didn't find what you were looking for? Ask a question
Top Posters
Since Sunday
N
8
1
1
Home Q & A Board Biology-Related Homework Help Cell Biology
New Topic  
barry barry
wrote...
Posts: 11630
Rep: 151 1
13 years ago
Briefly contrast the dideoxy DNA sequencing developed by Fred Sanger with pyrosequencing methods.
Source  iGenetics: A Molecular Approach, 3e (Russell/Bose)
Read 1006 times
1 Reply

Related Topics

Replies
wrote...
#1
Staff Member
13 years ago
Dideoxy DNA sequencing involves use of altered ( dideoxy) nucleotides that cause termination of replication at each site where such a nucleotide is inserted. The pyrosequencing method does not require chain termination. All four deoxynucleotide precursors are present at all times in the dideoxy sequencing reaction, whereas, only one is present at a time in the pyrosequencing reaction. The DNA template is immobilized on a bead in pyrosequencing, and the method of detection is the production of light by utilizing the pyrophosphate produced after the incorporation of a nucleotide. In the dideoxy method, the DNA template is not immobilized and the sequence is read with the help of fluorescent dyes attached to the dideoxy nucleotide molecules.
- Master of Science in Biology
- Bachelor of Science
New Topic      
Quick Reply
Bold
Italic
Underline
Strikethrough
Subscript
Superscript
Insert Math Equation
Font Name
Font Color
Align left
Center
Align right
Bullet list
Numbered list
Insert a horizontal rule
Insert a table
Insert a Quote
View source
Explore
Post your homework questions and get free online help from our incredible volunteers
  298 People Browsing
Start New Topic Take the Tour CoursesNew Study Tools Topics Trending Browse by Textbook
Live Chat
Show Emoticons
:):(;):P:D:|:O:?:nerd:8o:glasses::-):-(:-*O:-D>:-D:o):idea::important::help::error::warning::favorite:
Related Images
  
 689
  
 709
  
 5878
Your Opinion
Which is the best fuel for late night cramming?
Votes: 512
Previous poll results: Where do you get your textbooks?