× Didn't find what you were looking for? Ask a question
Top Posters
Since Sunday
Home Q & A Board Biology-Related Homework Help Genetics and Developmental Biology
New Topic  
CooperS CooperS
wrote...
Posts: 63
Rep: 3 0
9 years ago
Hi,

When you sequence DNA, for example by Sanger sequencing, do you normally replicate the unknown sample first to obtain cDNA and then sequence that? This way the DNA products you get will tell you the sequence of the gene you were looking for. If you didn't replicate your unknown DNA first, you'd be sequencing the complimentary sequence. This isn't a big deal, because you could just deduce its compliment, but I was just wondering what scientists normally do.

Thanks,
Cooper
Read 354 times
1 Reply
~Cooper

Related Topics

Replies
wrote...
#1
9 years ago
Hello, CooperS, see if this helps - https://biology-forums.com/index.php?topic=4151
Biology - The only science where multiplication and division mean the same thing.
New Topic      
Quick Reply
Explore
Post your homework questions and get free online help from our incredible volunteers
  1238 People Browsing
Start New Topic Take the Tour CoursesNew Study Tools Topics Trending Browse by Textbook
Related Images
  
 167
  
 43
  
 29
Your Opinion
Who's your favorite biologist?
Votes: 586
Previous poll results: Who will win the 2024 president election?