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Home Q & A Board Biology-Related Homework Help Biochemistry
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juliabreslin juliabreslin
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12 years ago
2. How is cutting with the scissors different from cleaving with a restriction enzyme?



3. Enzymes that modify DNA, such as restriction enzymes, have been discovered and isolated from living cells. What function do you think they have in living cells?
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12 years ago
Quote from: juliabreslin (12 years ago)
2. How is cutting with the scissors different from cleaving with a restriction enzyme?

Blunt vs. Sticky Ends - restriction enzymes can cut DNA sequences in these two different methods

a. Blunt ends - the DNA fragments produced have even, straight-cut ends = like a scissors cut.

HpaI
5' GTT ||| AAC 3'
3' CAA ||| TTG 5'

b. Sticky ends - the DNA fragments produced have uneven, overlapping ends; this "cut and paste" technology allowed for the beginnings of Recombinant DNA Applications

EcoRI HindIII
5' G ||| AATTC 3' 5' A ||| AGCTT 3'
3' CTTAA ||| G 5' 3' TTCGA ||| A 5'

Blunt ends are like a scissors cut and sticky ends are not.
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12 years ago
3. maybe for protectionj against virus when injecting its genetic material in to host
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goodasitgetsgoodasitgets
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12 years ago
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