Top Posters
Since Sunday
6
y
2
m
2
m
2
u
2
m
2
B
2
M
2
e
2
k
2
N
2
y
2
Home Q & A Board Biology-Related Homework Help Biochemistry
New Topic  
juliabreslin juliabreslin
wrote...
Go to Answer
Posts: 96
Rep: 3 0
12 years ago
2. How is cutting with the scissors different from cleaving with a restriction enzyme?



3. Enzymes that modify DNA, such as restriction enzymes, have been discovered and isolated from living cells. What function do you think they have in living cells?
Read 3473 times
3 Replies
Replies
wrote...
#1
Donated
Trusted Member
12 years ago
Quote from: juliabreslin (12 years ago)
2. How is cutting with the scissors different from cleaving with a restriction enzyme?

Blunt vs. Sticky Ends - restriction enzymes can cut DNA sequences in these two different methods

a. Blunt ends - the DNA fragments produced have even, straight-cut ends = like a scissors cut.

HpaI
5' GTT ||| AAC 3'
3' CAA ||| TTG 5'

b. Sticky ends - the DNA fragments produced have uneven, overlapping ends; this "cut and paste" technology allowed for the beginnings of Recombinant DNA Applications

EcoRI HindIII
5' G ||| AATTC 3' 5' A ||| AGCTT 3'
3' CTTAA ||| G 5' 3' TTCGA ||| A 5'

Blunt ends are like a scissors cut and sticky ends are not.
wrote...
#2
12 years ago
3. maybe for protectionj against virus when injecting its genetic material in to host
Answer accepted by topic starter
goodasitgetsgoodasitgets
wrote...
#3
Posts: 2
Rep: 2 0
12 years ago
Sign in or Sign up in seconds to unlock everything for free

Related Topics
New Topic      
Quick Reply
Explore
Post your homework questions and get free online help from our incredible volunteers
  545 People Browsing
Start New Topic Take the Tour CoursesNew Study Tools Topics Trending Browse by Textbook
Live Chat
Related Images
  
 378
  
 84
  
 167
Your Opinion
Who will win the 2024 president election?
Votes: 119
Closes: November 4
Previous poll results: How often do you eat-out per week?