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colleen colleen
wrote...
Valued Member
Posts: 17077
11 years ago
Normally, a certain enzyme has maximum activity at 37°C, but once it has been heated to 100°C and then cooled back down to 37°C, the enzyme is no longer active. Why?
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Sunshine ☀ ☼

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wrote...
11 years ago
The enzyme function is directed by its structure, which is governed by its amino acid content. The position of the amino acid is important too as the interaction between their side chain is responsible for the protein final conformation. The protein conformation is affected by heat (destruction of both the secondary and tertiary structures). When cold down the protein most likely will not get back to its normal conformation it needs proteins call chaperons or heat shock proteins. These proteins are included in your reaction
wrote...
Educator
11 years ago
The enzyme's three-dimensional shape has been distorted, and the active site is no longer able to bind the appropriate substrates in the reaction.
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