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Smitty Smitty
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11 years ago
While studying enzyme kinetics in the lab, a scientist observed that the rate of a reaction involving enzyme amylase increased as the concentration of starch increased. With further increase in starch concentration, the amylase activity did not change. Why did this happen?
A. The starch molecules did not bind to the enzyme amylase efficiently.
B. The enzyme amylase was saturated, as all its active sites were occupied by substrate starch.
C. The starch molecules were converted to product glucose as soon as they bound to enzyme amylase.
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11 years ago
B. This is because there were still active sites available for the starch substrate to bind to when the concentration was initially increased (the reaction will have been first order with respect to the concentration of starch). However, at a certain concentration, all the active sites of the enzymes will be full so there will be no active sites available for the starch molecules to bind to (the reaction is now zero order with respect to the concentration of starch).
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