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Home Q & A Board Biology-Related Homework Help Biochemistry
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flyfin flyfin
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11 years ago
Can anyone explain the sticky ends of a plasmid cleaved with PpuMI? What do the sticky ends look like? ;o)
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#1
11 years ago
Restriction endonuclease PpuMI recognizes the sequence "RGGWCCY" in double stranded DNA and cuts after the second position on each strand.
The ends of the restriction fragments resulting from digesting with PpuMI should be 'sticky' with a 5-prime overhang and looks like this.
Here ' / ' indicates the cut on the DNA strands by PpuMI.

5´-R G/G W C C Y-3´
3´-Y C C W G /G R-5´
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