. Help Charlie analyze his gel
A new lab technician, Charlie, was supposed to perform a restriction digest on the phage lambda DNA with BamHI enzyme. Charlie was in a hurry and, instead of BamHI, he used a DNA ligase, with the DNA ligase buffer. He also left a tube on the bench in the lab, where it remained during the weekend. When he came back next Monday morning, he noticed that it was quit cold in the lab. He also discovered that he’s left the lambda BamHI digest on the lab bench. Because the tube was left in a much colder temperature, Charlie thought that he needed to add more BamHI enzyme, which he did, and then placed the sample at 37°C. After giving his experiment some though, he decided that he had to repeat the digest because it was not properly done by the protocol. He set up the second digest but forgot to label the tube properly. Then, Charlie run both samples side by side on 0.8% agarose gel in TBE buffer, along with the ladder. After Charlie stained the gel he realize that he did not know which sample was which. To help Charlie,
analyze the pattern on the picture below and explain your conclusion. Use the table to support your conclusions. The size of the phage lambda DNA is 48,502 base pairs. It has five recognition sites for BamHI enzyme.

QUESTION: If Charlie, instead of the ligase buffer, added BamHI buffer to the tube he left over the weekend, would the patent on his gel look different? If so, in what way?
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