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florapt93 florapt93
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12 years ago
How is green fluorescent protein related to the understanding of necrosis?
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#1
12 years ago
GFP is used as a marker - so inherently, it has nothing to do with necrosis.  You can use a process called immunohistochemistry to label dying cells with GFP.  GFP is always something you have to you add (unless of course you are looking at the cells of a certain type of jellyfish or a transgenic animal - but I'm guessing you probably are not).  The result of this is seeing your labeled cells (dying cells) glowing green under the microscope when you shine a blue laser on them.  But there are much easier ways to see necrosis then GFP...
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