× Didn't find what you were looking for? Ask a question
Top Posters
Since Sunday
5
a
5
k
5
c
5
B
5
l
5
C
4
s
4
a
4
t
4
i
4
r
4
Home Q & A Board Science-Related Homework Help Physics
New Topic  
smmoh4 smmoh4
wrote...
Posts: 118
Rep: 4 0
11 years ago
what are the steps for lambda DNA library creation  in puc19 vectors?How will you design the experiment ? On what basis we select 1 or 2 enzymes to digest both lambda phage DNA and puc19 plasmid DNA
Read 292 times
1 Reply

Related Topics

Replies
wrote...
#1
11 years ago
When you are creating a library, you are basically cloning all the required DNA in fragments.

If you select puc19 as a vector, you must ensure in your selection of restriction enzymes that both the DNA and the vector have the same restriction sites i.e. cut sites. Furthermore, you must make sure that the restriction sites are far apart enough to create fragments of the proper size for the vector.
New Topic      
Quick Reply
Explore
Post your homework questions and get free online help from our incredible volunteers
  1259 People Browsing
Start New Topic Take the Tour CoursesNew Study Tools Topics Trending Browse by Textbook
Live Chat
Related Images
  
 9
  
 300
  
 137
Your Opinion
Which of the following is the best resource to supplement your studies:
Votes: 292
Previous poll results: What's your favorite funny biology word?