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minjai0322 minjai0322
wrote...
13 years ago
When dinitrophenol (DNP) is added to mitochondria, the inner membrane becomes permeable to protons.
(a) How will the electrochemical proton gradient change in response to DNP?
(b) DNP was one described as a weight loss drug. How would it promote weight loss and why do you think it is no longer prescribed?
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wrote...
Educator
13 years ago
a.  DNP will collapse the electrochemical proton gradient completely.  Protons pumped across the inner membrane will be carried back across the membrane by DNP, therefore no energy can be stored across the membrane. 

b.  K+ ions will be driven into the matrix by the electrical potential of the inner membrane (negative inside, positive outside).  The influx of positively charges K+ ions will abolish the membrane's electrical potential without affecting the concentration component of the proton gradient (the pH difference).  As a result, only part of the driving force that makes it energetically favorable for protons to flow back into the matrix will be lost.

Bio_man 8)
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