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Home Q & A Board Biology-Related Homework Help General Biology
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R3N-R3N R3N-R3N
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11 years ago
Part B - Inserting the vgp gene into a cloning vector
In the second step of the cloning procedure, DNA fragments produced by a restriction enzyme are inserted into a cloning vector that has been cut by the same enzyme. The vector used in this tutorial is a plasmid.
To clone the vgp gene into the plasmid below, a restriction enzyme must do all of the following:
cut the human DNA on both sides of the vgp gene, but not inside the gene
cut the plasmid without cutting inside the ampicillin resistance gene (ampR ), shown in blue (The importance of the ampR gene will be explained in Part C.)
produce sticky ends when it cuts both the human DNA and the plasmid, so that the human DNA fragments can combine with the plasmid

The questions below will help determine which restriction enzyme should be used to make a plasmid that contains both an intact vgp gene and a functional ampR gene.
Answer questions 1–4 by selecting from the answer choices on the left. Drag the correct answer to the right of each question.
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#1
11 years ago
a) BamHI - Yes, produces sticky ends, and cuts around the gene
b) HindIII - Yes, produces sticky ends, and cuts around the gene
c) EcoRI - No, produces sticky ends, but cuts IN the gene
d) HaeIII - No, produces blunt ends, but does cut around the gene
sborjas,  captainamerica2,  khuie2017
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ochoriosochorios
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#2
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11 years ago
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This verified answer contains over 160 words and 1 attached file(s).
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bio_man,  Zetsubou,  daveev,  wyki,  mrsalvarado07,  miketheg,  Nashpati,  Violet739,  padre,  annabelle

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wrote...
#3
11 years ago
1)BamHI , Haelll and HindIIl
2) BamHI, EcoRI, and HaeIII,
3) BamHI, EcoRI, and HindIII,
4) BamHI only.

Just finish my home work You can trust this answer Kissing Face with Closed Eyes
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R3N-R3N Author
wrote...
#4
11 years ago
Thank you so much ochorios and our guest George both of you were correct.. both of you are life savers..Bio has been killing me Frowning Face
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#5
11 years ago
noooo problem Smiling Face with Open Mouth and Tightly-closed Eyes
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#6
10 years ago
Excellent, thank you!!!
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#7
10 years ago
Thanks for the help guys and the explanation really clarified everything
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#8
10 years ago
Thank you ♥
wrote...
#9
4 years ago
GREAT THANK YOU
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