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mgbdd mgbdd
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10 years ago
Although your instructor worked quickly to transfer a sample of bacteria expressing rfp, there is a good chance that some non-rfp-expressing bacteria were transferred as well.  What would prevent the growth of these bacteria in the LB/amp/ara broth?  The bacteria was exposed to a recombinant DNA called pARA-R.  What would prevent the growth of the non-rfp-expressing bacteria in the LB/amp/ara broth but not the rfp-expressing bacteria?
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wrote...
10 years ago
the ampicillin should kill any bacteria not expressing the recombinant DNA.
The recombinant DNA has a gene on it responsible for encoding resistance to ampicillin and other bacteria will not have this DNA or the gene and therefore will be destroyed by the amp.
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