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Home Q & A Board Biology-Related Homework Help Genetics and Developmental Biology
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fodog414 fodog414
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11 years ago
Thalassemia is a recessive blood disease caused by reduced synthesis of one of the globin genes. While SCD is a mutation in the structure of hemoglobin, thalassemias can also be caused by mutations in the regulatory elements, causing too little globin to be produced and resulting in anemia. You've designed a DNA probe that you want to use to screen a family for a rare mutation in the β-globin gene that causes thalassemia. First you will use the probe for northern blotting. What information will the northern blot tell you?

   
whether the mRNA for β-globin is being translated into functional proteins in a given cell population
   
the size of the mRNA being transcribed
   
whether the amount of the β-globin gene transcript is within normal range, and the size of the mRNA gene product
   
whether the amount of the β-globin gene being transcribed (mRNA levels) is within normal range
   
the presence of a particular mutation by restriction fragment analysis
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#1
11 years ago
something that will help is using the "snow drop"  Typically you wont really get any questions that will require much more info on this topic.

S dNA
N rNA
O
W pROTEINS
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fodog414 Authorfodog414
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Posts: 279
Rep: 26 0
11 years ago
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