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dumakey1212 dumakey1212
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11 years ago
For a lab, I had four different colored leaves, a green, yellow, red, and orange one. I crushed the leaves up and prepared chloroplast pigment extracts for each of the four leaves. Each of the pigments was smeared onto different paper strips to place in the solvent and allow the chromatography process to happen.

The results seemed inconclusive. I was expecting for the green leaf sample to have the green band travel furthest because this is the chlorophyll pigment. I was expecting the orange and yellow leaves to have the yellow and orange bands travel the furthest. Is this thinking correct or am I missing something? How can I use known Rf values of pigments to determine if they match up with the results I got from the experiment?

Thanks!
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11 years ago
Interesting question. I think this video might help (Downwards Arrow) along with the attachment.
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Mastering in Nutritional Biology
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