Bacterial Growth Curve

HI micro

I totally missed your thread. What did you end up discovering here.

Sorry about that.

Nothing so far

If there is nothing in the literature about the replication time for the bacteria, here is what I would do.

First inoculate a flask from a colony and let it grow overnight.  The next day you will have a flask that is in lag phase.  Next I would dilute the starter culture to a range of concentrations.  For instance, start 4.95mL of media, then add 50uL of the o/n culture,  4.9mL media and 100uL of the on/ culture.  I would do a series of 50uL, 100uL, 250uL, 500uL, and 1mL of the o/n culture to enough media to equal 5mL.  Then check each dilution by spec periodically to see the growth and write them down.  One of those will assure you get a low enough starting concentration to see the growth curve without having to spend extra time in lab cause you diluted to too low of an initial starting concentration.

Hope this helps!