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Samples of the DNA separated by gel electrophoresis

Description
In the Southern blotting technique, samples of the DNA to be probed are cut with restriction enzymes and the fragments are separated by gel electrophoresis.

i 2 3 l. DNA samples cut with — — — restriction enzymes are loaded on ugerese gel lor electrophoresis Lane 1: DNA size markers Lane NA cut with restriction enzyme A Lane . NA cut with restriction enzyme 5 2. DNA is separaled by electrophoresis l 2 3 Weight _ Pa er towels I p __ DNA-binding liner .- - I DNA ls denatured _ —- Gel is placed "' one on ewlck _ I u P 9 Gel Wick (sponge) Butter a. DNA-binding liner, paper towels. and weight are placed on gel; buffer passes upward lhrough sponge by capillary action, lranslerring DNA lragrrrems to lilter Radioactive or nonradioacuvely labeled probe / 4. The liller is placed in heat-sealed bag or a tube with solulien containing labeled probe; probe hybr es with complementary sequences l _> _ — \\ _ _ 5. Bound probe All size markers appear because deleoleo by film or they are labeled; in lanes 2 PW” slallal captured and 3, only those bands that hybridize Wilh fl digi‘fl' came“ with probe are visible
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